Growth of Azotobacter vinelandii UWD in Fish Peptone Medium and Simplified Extraction of Poly-β-Hydroxybutyrate
نویسندگان
چکیده
منابع مشابه
Control of polyhydroxyalkanoate synthesis in Azotobacter vinelandii strain UWD
Microbiology, University of Alberta, Edmonton, Alberta T6G 2E9, Canada polyhydroxyalkanoate (PHA) in mutant Azotobacter vinelandii UWD, the kinetic properties of 3-ketothiolase, acetoacetyl-CoA reductase, and /3hydroxybutyrate dehydrogenase were examined. The regulatiorn of the condensation of acetyl-CoA mediated by 3-ketothiolase was narmal, in that it was negatively regulated by free CoA, but...
متن کاملImmunostimulatory and growth-promoting potential of poly-β-hydroxybutyrate in rainbow trout (Oncorhynchus mykiss) fingerlings culture
In the current study, the effect of dietary supplementation of poly-β-hydroxybutyrate (PHB) on growth performance, digestive enzymes activity, body composition, non-specific immune response and diseases resistance of rainbow trout fingerlings were investigated. Five hundred and fifty-two healthy fingerlings (8.2±1.0 g) were randomly distributed in 12 tanks (150 L) at a density of 46 fish/ tank ...
متن کاملThe GacS sensor kinase regulates alginate and poly-beta-hydroxybutyrate production in Azotobacter vinelandii.
Azotobacter vinelandii produces two polymers: the extracellular polysaccharide alginate and the intracellular polyester poly-beta-hydroxybutyrate (PHB). A cosmid clone (pSMU588) from an A. vinelandii gene library diminished alginate production by A. vinelandii mucoid strain ATCC 9046. The nucleotide sequence and predicted amino acid sequence of the locus responsible for the mucoidy suppression ...
متن کاملCloning of poly (3-Hydroxybutyrate) synthesis genes from Azotobacter vinelandii into Escherichia coli
The Azotobacter vinelandii is an aerobic, nitrogen-fixing bacterium with the ability to accumulate polyesters such as poly (3-hydroxybutyrate), a particular group of the polyhydroxyalkanoates (pha), when will be cultivated on several carbon sources, including sucrose. Although the capacity of Azotobacter strains to accumulate polyesters is well known, and the genes responsible for their synthes...
متن کاملCytokinins in Azotobacter vinelandii Culture Medium.
Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentatively identified as trans-zeatin, isopentenyladenosine, and isopentenyladenine. T...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1993
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.59.12.4236-4244.1993